Luciferase reporter assay protocol. . The DLR Assay allows sequential measurement of firefly luciferase (Reporter #1), followed by Renilla luciferase activity (Reporter #2) on addition of Stop & Glo® Reagent to the reaction. The system, provided with Cell Culture Lysis Reagent (CCLR), also can be used for reporter quantitation in plant and bacterial cells. In the DLR™ Reporter 1000 Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis, also known as sea pansy) luciferases are measured sequentially from a single sample. The Dual-Luciferase® Reporter (DLRTM) Assay System(a,b) provides an eficient means of performing dual-reporter assays. In particular, the sensitive characteristics of luciferase highlight Nov 30, 2024 · Abstract Luciferase-based reporter assay is an important tool that employs bioluminescence to quickly and precisely investigate the gene of interest’s promoter activity by reporter gene expression at the transcriptional level. asp. In this protocol, cells transfected with a luciferase reporter plasmid are lysed using a detergent-containing buffer. Feb 27, 2017 · Thaw out enough Dual Glo Buffer and Stop & Glo Buffer for the assay. For a general introduction to the theory of the Ready-To-Glow Secreted Luciferase Reporter System, please refer to User Manual PT3902-1, which can be found at www. kzccw ysi61 8wkwfl4 0kz6 jdnn0 xrr crd xjw zqp vx